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This study was conducted to investigate the tuber storage protein patterns and expressions when axillary buds (tuberization sink) were removed from in vitro potato (Solanum tuberosum L. cv. Daeji) axillary bud culture. The effects of various growth regulators on the protein changes, especially, gene expression of patatin and 22-kDa Kunitz-type potato proteinase inhibitor (22-kDa KPPI) in the shoot of in vitro potato plantlets cultured on MS medium (MS + Sucrose 80 g·L^(-1) + agar 8 g·L^(-1)) added with various growth regulators (CCC 250 ㎎·L^(-1), ABA 0.01 ㎎·L^(-1), or GA 5 ㎎·L^(-1)), were investigated. In IEF~SDS-PAGE of proteins extracted from shoots with removed axillary bud of in vitro potato plantlets during 1 week, protein patterns differed in the molecular weights of 22-kDa to 60-kDa proteins. In immunoblotting for 22-kDa KPPI of shoot with removed axillary buds, 22-kDa KPPI specific antibody was reacted with proteins from shoot of CCC 250 ㎎·L^(-1), ABA 0.01 ㎎·L^(-1), and control treatment, but not reacted with proteins from shoots cultured on GA, 5 ㎎·L^(-1). In immunoblot analysis of patatin, the proteins extrated from shoot with removed axillary buds for 1 or 3 weeks culture, patatin antibody was reacted with proteins from all shoots, regardless of the treatments of plant growth regulators. From these results, the 22-kDa KPPI could be a candidate marker for the identification of the tuberization or nontuberization of potato plantlets grown in vitro condition.
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